Although much has been written about stress and well-being in the workplace, research and theory in this area have been limited by an almost complete emphasis on individual-level processes. This chapter addresses this limitation by exploring the impact of organizational climate and culture on stress and well-being in organizational settings. The basic thesis proposed in this chapter is that climate and culture can affect the stress process both directly and indirectly, and can either exacerbate the effect of workplace stressors or act as a source of resilience to employees. The chapter begins by briefly defining what is meant by “stress,” “well-being,” “organizational climate,” and “organizational culture.” This is important because all of these terms have been confused and frequently misused, even in the research literature. It then examines the various ways that climate and culture may affect the stress and well-being of employees, along with empirical examples of these. The chapter concludes with a discussion of the practical implications of the impact of climate and culture, and provides a number of suggestions for future research.