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date: 15 June 2021

Abstract and Keywords

This article considers the use of microspectroscopy for discriminating nanomolecular cellular alterations in biomedical research. It begins with an overview of some existing mid-infrared microspectroscopy techniques, including FTIR microspectroscopy and Raman microspectroscopy. It then discusses near-field techniques such as scanning near-field optical microscopy, near-field Raman microscopy, and photothermal microspectroscopy (PTMS). It also examines promising alternative sources of IR light, possible advantages of using normal atomic force microscopy probes, experimental procedures for PTMS, and prospects for high spatial resolution in near-field FTIR spectroscopy. Finally, it describes the spectroscopic detection of small particles, along with the use of the analysis paradigm to discriminate nanomolecular cellular alterations in biomedical research.

Keywords: nanomolecular cellular alterations, biomedical research, mid-infrared microspectroscopy, FTIR microspectroscopy, Raman microspectroscopy, scanning near-field optical microscopy, near-field Raman microscopy, photothermal microspectroscopy, atomic force microscopy, small particles

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